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By NASA
4 min read
Preparations for Next Moonwalk Simulations Underway (and Underwater)
The biofilm mitigation research team at NASA’s Marshall Space Flight Center assembled its own test stand to undertake a multi-month assessment of a variety of natural and chemical compounds and strategies for eradicating biofilm accretion caused by bacteria and fungi in the wastewater tank assembly on the International Space Station. Testing will help NASA extend the lifecycle of water reclamation and recycling hardware and ensure astronauts can sustain clean, healthy water supplies on long-duration missions in space and on other worlds.NASA/Eric Beitle A small group of scientists on the biofilm mitigation team at NASA’s Marshall Space Center in Huntsville, Alabama, study solutions to combat the fast-growing colony of bacteria or fungi, known as biofilm, for future space missions.
Biofilm occurs when a cluster of bacteria or fungi generates a slimy matrix of “extracellular polymeric substances” to protect itself from adverse environmental factors. Biofilm can be found nearly anywhere, from the gray-green scum floating on stagnant pond water to the pinkish ring of residue in a dirty bathtub.
For medical, food production, and wastewater processing industries, biofilm is often a costly issue. But offworld, biofilm proves to be even more resilient.
“Bacteria shrug off many of the challenges humans deal with in space, including microgravity, pressure changes, ultraviolet light, nutrient levels, even radiation,” said Yo-Ann Velez-Justiniano, a microbiologist and environmental control systems engineer at Marshall.
Biofilm is icky, sticky – and hard to kill.
Liezel Koellner
Chemical Engineer and NASA Pathways Intern
“Biofilm is icky, sticky – and hard to kill,” said Liezel Koellner, a chemical engineer and NASA Pathways intern from North Carolina State University in Raleigh. Koellner used sophisticated epifluorescence microscopy, 3D visualizations of 2D images captured at different focal planes, to fine-tune the team’s studies.
Keenly aware of the potential hurdles biofilm could pose in future Artemis-era spacecraft and lunar habitats, NASA tasked engineers and chemists at Marshall to study mitigation techniques. Marshall built and maintains the International Space Station’s ECLSS (Environment Control and Life Support System) and is developing next-generation air and water reclamation and recycling technologies, including the system’s wastewater tank assembly.
“The wastewater tank is ‘upstream’ from most of our built-in water purification methods. Because it’s a wastewater feed tank, bacteria and fungus grow well there, generating enough biofilm to clog flow paths and pipes along the route,” said Eric Beitle, ECLSS test engineer at Marshall.
To date, the solution has been to pull and replace old hardware once parts become choked with biofilm. But engineers want to avoid the need for such tactics.
“Even with the ability to 3D-print spare parts on the Moon or Mars, it makes sense to find strategies that prevent biofilm buildup in the first place,” said Velez-Justiniano.
The team took the first step in June 2023 by publishing the complete genome sequence of several strains of bacteria isolated from the space station’s water reclamation system, all of which cultivate biofilm formation.
They next designed a test stand simulating conditions in the wastewater tank about 250 miles overhead, which permits simultaneous study of multiple mitigation options. The rig housed eight Centers for Disease Control and Prevention biofilm reactors – cylindrical devices roughly the size of a runner’s water bottle – each 1/60th the size of the actual tank.
Yo-Ann Velez-Justiniano, left, and Connor Murphy, right, both Environmental Control and Life Support Systems engineers at NASA’s Marshall Space Flight Center in Huntsville, Alabama, prepare slides for study of cultured bacterial biofilm in the center’s test facility NASA/Eric Beitle Each bioreactor holds up to 21 unique test samples on slides, bathed continuously in a flow of real or ersatz wastewater, timed and measured by the automated system, and closely monitored by the team. Because of the compact bioreactor size, the test stand required 2.1 gallons of ersatz flow per week, continuously trickling 0.1 milliliters per minute into each of the eight bioreactors.
“Essentially, we built a collection of tiny systems that all had to permit minute changes to temperature and pressure, maintain a sterile environment, provide autoclave functionality, and run in harmony for weeks at a time with minimal human intervention,” said Beitle. “One phase of the test series ran nonstop for 65 days, and another lasted 77 days. It was a unique challenge from an engineering perspective.”
Different surface mitigation strategies, upstream counteragents, antimicrobial coatings, and temperature levels were introduced in each bioreactor. One promising test involved duckweed, a plant already recognized as a natural water purification system and for its ability to capture toxins and control wastewater odor. By devouring nutrients upstream of the bioreactor, the duckweed denied the bacteria what it needs to thrive, reducing biofilm growth by up to 99.9%.
Over the course of the three-month testing period, teams removed samples from each bioreactor at regular intervals and prepared for study under a microscope to make a detailed count of the biofilm colony-forming units on each plate.
“Bacteria and fungi are smart,” said Velez-Justiniano. “They adapt. We recognize that it is going to take a mix of effective biofilm mitigation methods to overcome this challenge.”
Biofilm poses as an obstacle to long-duration spaceflight and extended missions on other worlds where replacement parts may be costly or difficult to obtain. The biofilm mitigation team continues to assess and publish findings, alongside academic and industry partners, and will further their research with a full-scale tank experiment at Marshall. They hope to progress to flight tests, experimenting with various mitigation methods in real microgravity conditions in orbit to find solutions to keep surfaces clean, water potable, and future explorers healthy.
Joel Wallace
Marshall Space Flight Center, Huntsville, Ala.
256-786-0117
joel.w.wallace@nasa.gov
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Last Updated Jul 09, 2024 EditorBeth RidgewayLocationMarshall Space Flight Center Related Terms
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By European Space Agency
Video: 00:38:43 Australian Space Agency astronaut candidate Katherine Bennell-Pegg joined ESA’s astronaut candidates from the class of 2022 for basic training through a cooperation agreement with ESA. Tune in as she shares her experiences in astronaut training, her favourite lessons, and what keeps her inspired on her journey to the stars!
This is episode 7 of our ESA Explores podcast series introducing the ESA astronaut class of 2022, recorded in March 2024.
Find out more about the ESA astronaut class of 2022.
Access all ESA Explores podcasts.
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By NASA
3 Min Read July’s Night Sky Notes: A Hero, a Crown, and Possibly a Nova!
Like shiny flakes sparkling in a snow globe, over 100,000 stars whirl within the globular cluster M13, one of the brightest star clusters visible from the Northern Hemisphere. Located 25,000 light-years from Earth with an apparent magnitude of 5.8, this glittering metropolis of stars in the constellation Hercules can be spotted with a pair of binoculars most easily in July. Credits:
NASA by Vivan White of the Astronomical Society of the Pacific
High in the summer sky, the constellation Hercules acts as a centerpiece for late-night stargazers. At the center of Hercules is the “Keystone,” a near-perfect square shape between the bright stars Vega and Arcturus that is easy to recognize and can serve as a guidepost for some amazing sights. While not the brightest stars, the shape of the hero’s torso, like a smaller Orion, is nearly directly overhead after sunset. Along the edge of this square, you can find a most magnificent jewel – the Great Globular Cluster of Hercules, also known as Messier 13.
Look up after sunset during summer months to find Hercules! Scan between Vega and Arcturus, near the distinct pattern of Corona Borealis. Once you find its stars, use binoculars or a telescope to hunt down the globular clusters M13 (and a smaller globular cluster M92). If you enjoy your views of these globular clusters, you’re in luck – look for another great globular, M3, near the constellation Boötes. Credit: Stellarium Globular clusters are a tight ball of very old stars, closer together than stars near us. These clusters orbit the center of our Milky Way like tight swarms of bees. One of the most famous short stories, Nightfall by Isaac Asimov, imagines a civilization living on a planet within one of these star clusters. They are surrounded by so many stars so near that it is always daytime except for once every millennium, when a special alignment (including a solar eclipse) occurs, plunging their planet into darkness momentarily. The sudden night reveals so many stars that it drives the inhabitants mad.
Back here on our home planet Earth, we are lucky enough to experience skies full of stars, a beautiful Moon, and regular eclipses. On a clear night this summer, take time to look up into the Keystone of Hercules and follow this sky chart to the Great Globular Cluster of Hercules. A pair of binoculars will show a faint, fuzzy patch, while a small telescope will resolve some of the stars in this globular cluster.
A red giant star and white dwarf orbit each other in this animation of a nova similar to T Coronae Borealis. The red giant is a large sphere in shades of red, orange, and white, with the side facing the white dwarf the lightest shades. The white dwarf is hidden in a bright glow of white and yellows, which represent an accretion disk around the star. A stream of material, shown as a diffuse cloud of red, flows from the red giant to the white dwarf. When the red giant moves behind the white dwarf, a nova explosion on the white dwarf ignites, creating a ball of ejected nova material shown in pale orange. After the fog of material clears, a small white spot remains, indicating that the white dwarf has survived the explosion. NASA/Goddard Space Flight Center Bonus! Between Hercules and the ice-cream-cone-shaped Boötes constellation, you’ll find the small constellation Corona Borealis, shaped like the letter “C.” Astronomers around the world are watching T Coronae Borealis, also known as the “Blaze Star” in this constellation closely because it is predicted to go nova sometime this summer. There are only 5 known nova stars in the whole galaxy. It is a rare observable event and you can take part in the fun! The Astronomical League has issued a Special Observing Challenge that anyone can participate in. Just make a sketch of the constellation now (you won’t be able to see the nova) and then make another sketch once it goes nova.
Tune into our mid-month article on the Night Sky Network page, as we prepare for the Perseids! Keep looking up!
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By European Space Agency
Video: 00:24:06 John McFall, a member of the European astronaut reserve from the ESA astronaut class of 2022, brings a diverse background to his role. With experience as an orthopaedic and trauma surgeon and a former Paralympic sprinter, John is participating in the groundbreaking "Fly!" feasibility study. This initiative seeks to enhance our comprehension of the challenges posed by space flight for astronauts with physical disabilities, aiming to overcome these barriers. Tune in to discover more about John and the "Fly!" project.
This is Episode 9 of our ESA Explores podcast series, delving into everything you want to know about the ESA astronaut class of 2022. Recorded in November 2023.
Find out more about John.
About the ESA astronaut class of 2022.
Hosted by Laura Zurmühlen, with audio editing and music by Denzel Lorge, and cover art by Gaël Nadaud.
Access all ESA Explores podcasts.
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By NASA
2 min read
June’s Night Sky Notes: Constant Companions: Circumpolar Constellations, Part III
by Kat Troche of the Astronomical Society of the Pacific
In our final installment of the stars around the North Star, we look ahead to the summer months, where depending on your latitude, the items in these circumpolar constellations are nice and high. Today, we’ll discuss Cepheus, Draco, and Ursa Major. These objects can all be spotted with a medium to large-sized telescope under dark skies.
From left to right: Ursa Major, Draco, and Cepheus. Credit: Stellarium Web Herschel’s Garnet Star: Mu Cephei is a deep-red hypergiant known as The Garnet Star, or Erakis. While the star is not part of the constellation pattern, it sits within the constellation boundary of Cepheus, and is more than 1,000 times the size of our Sun. Like its neighbor Delta Cephei, this star is variable, but is not a reliable Cepheid variable. Rather, its brightness can vary anywhere between 3.4 to 5.1 in visible magnitude, over the course of 2-12 years.
This composite of data from NASA’s Chandra X-ray Observatory and Hubble Space Telescope gives astronomers a new look for NGC 6543, better known as the Cat’s Eye nebula. This planetary nebula represents a phase of stellar evolution that our sun may well experience several billion years from now. Credit: X-ray: NASA/CXC/SAO; Optical: NASA/STScI The Cat’s Eye Nebula: Labeled a planetary nebula, there are no planets to be found at the center of this object. Observations taken with NASA’s Chandra X-ray Observatory and Hubble Space Telescopes give astronomers a better understanding of this complex, potential binary star, and how its core ejected enough mass to produce the rings of dust. When searching for this object, look towards the ‘belly’ of Draco with a medium-sized telescope.
NASA’s Spitzer, Hubble, and Chandra space observatories teamed up to create this multi-wavelength view of the M82 galaxy. The lively portrait celebrates Hubble’s “sweet sixteen” birthday .X-ray data recorded by Chandra appears in blue; infrared light recorded by Spitzer appears in red; Hubble’s observations of hydrogen emission appear in orange, and the bluest visible light appears in yellow-green. Credit: NASA, ESA, CXC, and JPL-Caltech Bode’s Galaxy and the Cigar Galaxy: Using the arrow on the star map, look diagonal from the star Dubhe in Ursa Major. There you will find Bode’s Galaxy (Messier 81) and the Cigar Galaxy (Messier 82). Sometimes referred to as Bode’s Nebula, these two galaxies can be spotted with a small to medium-sized telescope. Bode’s Galaxy is a classic spiral shape, similar to our own Milky Way galaxy and our neighbor, Andromeda. The Cigar Galaxy, however, is known as a starburst galaxy type, known to have a high star formation rate and incredible shapes. This image composite from 2006 combines the power of three great observatories: the Hubble Space Telescope imaged hydrogen in orange, and visible light in yellow green; Chandra X-Ray Observatory portrayed X-ray in blue; Spitzer Space Telescope captured infrared light in red.
Up next, we celebrate the solstice with our upcoming mid-month article on the Night Sky Network page through NASA’s website!
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