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NASA: Life Signs Could Survive Near Surfaces of Enceladus and Europa


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NASA: Life Signs Could Survive Near Surfaces of Enceladus and Europa

Europa, a moon of Jupiter, and Enceladus, a moon of Saturn, have evidence of oceans beneath their ice crusts. A NASA experiment suggests that if these oceans support life, signatures of that life in the form of organic molecules (e.g. amino acids, nucleic acids, etc.) could survive just under the surface ice despite the harsh radiation on these worlds. If robotic landers are sent to these moons to look for life signs, they would not have to dig very deep to find amino acids that have survived being altered or destroyed by radiation.

“Based on our experiments, the ‘safe’ sampling depth for amino acids on Europa is almost 8 inches (around 20 centimeters) at high latitudes of the trailing hemisphere (hemisphere opposite to the direction of Europa’s motion around Jupiter) in the area where the surface hasn’t been disturbed much by meteorite impacts,” said Alexander Pavlov of NASA’s Goddard Space Flight Center in Greenbelt, Maryland, lead author of a paper on the research published July 18 in Astrobiology. “Subsurface sampling is not required for the detection of amino acids on Enceladus – these molecules will survive radiolysis (breakdown by radiation) at any location on the Enceladus surface less than a tenth of an inch (under a few millimeters) from the surface.”

The frigid surfaces of these nearly airless moons are likely uninhabitable due to radiation from both high-speed particles trapped in their host planet’s magnetic fields and powerful events in deep space, such as exploding stars. However, both have oceans under their icy surfaces that are heated by tides from the gravitational pull of the host planet and neighboring moons. These subsurface oceans could harbor life if they have other necessities, such as an energy supply as well as elements and compounds used in biological molecules.

Fountains of ice erupt from the surface of Enceladus. The small moon is mostly in shadow, which makes the geysers illuminated by sunlight stand out.
Dramatic plumes, both large and small, spray water ice and vapor from many locations along the famed “tiger stripes” near the south pole of Saturn’s moon Enceladus.
NASA/JPL/Space Science Institute

The research team used amino acids in radiolysis experiments as possible representatives of biomolecules on icy moons. Amino acids can be created by life or by non-biological chemistry. However, finding certain kinds of amino acids on Europa or Enceladus would be a potential sign of life because they are used by terrestrial life as a component to build proteins. Proteins are essential to life as they are used to make enzymes which speed up or regulate chemical reactions and to make structures. Amino acids and other compounds from subsurface oceans could be brought to the surface by geyser activity or the slow churning motion of the ice crust.

Europa has reddish brown lines and patches in this image from Juno.
This view of Jupiter’s icy moon Europa was captured by JunoCam, the public engagement camera aboard NASA’s Juno spacecraft, during the mission’s close flyby on Sept. 29, 2022. The picture is a composite of JunoCam’s second, third, and fourth images taken during the flyby, as seen from the perspective of the fourth image. North is to the left.

The images have a resolution of just over 0.5 to 2.5 miles per pixel (1 to 4 kilometers per pixel).

As with our Moon and Earth, one side of Europa always faces Jupiter, and that is the side of Europa visible here. Europa’s surface is crisscrossed by fractures, ridges, and bands, which have erased terrain older than about 90 million years.

Citizen scientist Kevin M. Gill processed the images to enhance the color and contrast.

NASA/JPL-Caltech/SwRI/MSSS Image processing: Kevin M. Gill CC BY 3.0

To evaluate the survival of amino acids on these worlds, the team mixed samples of amino acids with ice chilled to about minus 321 Fahrenheit (-196 Celsius) in sealed, airless vials and bombarded them with gamma-rays, a type of high-energy light, at various doses. Since the oceans might host microscopic life, they also tested the survival of amino acids in dead bacteria in ice. Finally, they tested samples of amino acids in ice mixed with silicate dust to consider the potential mixing of material from meteorites or the interior with surface ice.

Image showing samples loaded in a dewar before being chilled with liquid nitrogen.
This image shows experiment samples loaded in the specially designed dewar which will be filled with liquid nitrogen shortly after and placed under gamma radiation. Notice that the flame-sealed test tubes are wrapped in cotton fabric to keep them together because test tubes become buoyant in liquid nitrogen and start floating around in the dewar, interfering with the proper radiation exposure.
Candace Davison

The experiments provided pivotal data to determine the rates at which amino acids break down, called radiolysis constants. With these, the team used the age of the ice surface and the radiation environment at Europa and Enceladus to calculate the drilling depth and locations where 10 percent of the amino acids would survive radiolytic destruction.

Although experiments to test the survival of amino acids in ice have been done before, this is the first to use lower radiation doses that don’t completely break apart the amino acids, since just altering or degrading them is enough to make it impossible to determine if they are potential signs of life. This is also the first experiment using Europa/Enceladus conditions to evaluate the survival of these compounds in microorganisms and the first to test the survival of amino acids mixed with dust.

The team found that amino acids degraded faster when mixed with dust but slower when coming from microorganisms.

“Slow rates of amino acid destruction in biological samples under Europa and Enceladus-like surface conditions bolster the case for future life-detection measurements by Europa and Enceladus lander missions,” said Pavlov. “Our results indicate that the rates of potential organic biomolecules’ degradation in silica-rich regions on both Europa and Enceladus are higher than in pure ice and, thus, possible future missions to Europa and Enceladus should be cautious in sampling silica-rich locations on both icy moons.”

A potential explanation for why amino acids survived longer in bacteria involves the ways ionizing radiation changes molecules — directly by breaking their chemical bonds or indirectly by creating reactive compounds nearby which then alter or break down the molecule of interest. It’s possible that bacterial cellular material protected amino acids from the reactive compounds produced by the radiation.

The research was supported by NASA under award number 80GSFC21M0002, NASA’s Planetary Science Division Internal Scientist Funding Program through the Fundamental Laboratory Research work package at Goddard, and NASA Astrobiology NfoLD award 80NSSC18K1140.

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      The AVIRIS-3 sensor belongs to a line of imaging spectrometers built at JPL since 1986. The instruments have been used to study a wide range of phenomena — including fire — by measuring sunlight reflecting from the planet’s surface.
      During the March flights, researchers created three types of maps. One, called the Fire Quicklook, combines brightness measurements at three wavelengths of infrared light, which is invisible to the human eye, to identify the relative intensity of burning. Orange and red areas on the Fire Quicklook map show cooler-burning areas, while yellow indicates the most intense flames. Previously burned areas show up as dark red or brown.
      Another map type, the Fire 2400 nm Quicklook, looks solely at infrared light at a wavelength of 2,400 nanometers. The images are particularly useful for seeing hot spots and the perimeters of fires, which show brightly against a red background.
      A third type of map, called just Quicklook, shows burned areas and smoke.
      The Fire 2400 nm Quicklook was the “fan favorite” among the fire crews, said Ethan Barrett, fire analyst for the Forest Protection Division of the Alabama Forestry Commission. Seeing the outline of a wildfire from above helped Alabama Forestry Commission firefighters determine where to send bulldozers to stop the spread. 
      Additionally, FireSense personnel analyzed the AVIRIS-3 imagery to create digitized perimeters of the fires. This provided firefighters fast, comprehensive intelligence of the situation on the ground.
      That’s what happened with the Castleberry Fire. Having a clear picture of where it was burning most intensely enabled firefighters to focus on where they could make a difference — on the northeastern edge. 
      Then, two days after identifying Castleberry Fire hot spots, the sensor spotted a fire about 4 miles (2.5 kilometers) southwest of Perdido, Alabama. As forestry officials worked to prevent flames from reaching six nearby buildings, they noticed that the fire’s main hot spot was inside the perimeter and contained. With that intelligence, they decided to shift some resources to fires 25 miles (40 kilometers) away near Mount Vernon, Alabama.
      To combat one of the Mount Vernon fires, crews used AVIRIS-3 maps to determine where to establish fire breaks beyond the northwestern end of the fire. They ultimately cut the blaze off within about 100 feet (30 meters) of four buildings. 
      “Fire moves a lot faster than a bulldozer, so we have to try to get around it before it overtakes us. These maps show us the hot spots,” Barrett said. “When I get out of the truck, I can say, ‘OK, here’s the perimeter.’ That puts me light-years ahead.”
      AVIRIS and the Firesense Airborne Campaign are part of NASA’s work to leverage its expertise to combat wildfires using solutions including airborne technologies. The agency also recently demonstrated a prototype from its Advanced Capabilities for Emergency Response Operations project that will provide reliable airspace management for drones and other aircraft operating in the air above wildfires.
      NASA Helps Spot Wine Grape Disease From Skies Above California News Media Contacts
      Andrew Wang / Jane J. Lee
      Jet Propulsion Laboratory, Pasadena, Calif.
      626-379-6874 / 818-354-0307
      andrew.wang@jpl.nasa.gov / jane.j.lee@jpl.nasa.gov
      2025-058
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      Last Updated Apr 23, 2025 Related Terms
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