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Monitoring Microorganisms

A set of the International Space Station's main solar arrays, slightly obscuring the smaller roll-out solar arrays, and the Kibo laboratory module with its exposed facility, a research platform that hosts external experiments, are pictured 261 miles above the Pacific Ocean.
iss070e049644 (Dec. 30, 2023) — A set of the International Space Station's main solar arrays, slightly obscuring the smaller roll-out solar arrays, and the Kibo laboratory module with its exposed facility, a research platform that hosts external experiments, are pictured 261 miles above the Pacific Ocean.
Credits: NASA

Science in Space January 2024

Crew members on the International Space Station have a lot of company – millions of bacteria and other microbes. The human body contains 10 times more microbes than human cells, and bacteria and fungi grow in and on just about everything around us on Earth.

Most bacteria are harmless, and many are beneficial or even essential to human functioning and well-being. But microgravity can make some microbes more likely to cause disease and bacteria and fungi may affect the function of spacecraft systems, by, for example, corroding metal. These organisms also could contaminate other planetary bodies that spacecraft and humans land on.

Some microbes inevitably come along for the ride on crew members and cargo traveling to the space station, and it is important to identify and control those that may be harmful – especially in a closed environment like a spacecraft. Multiple investigations have tracked, identified, and analyzed the station’s tiniest residents to help keep crew members and equipment – and even other planets – safe from any potential threats.

A current investigation, ISS Boeing Antimicrobial Coating, tests surface coatings designed to inhibit the growth of microbes to protect crew members and equipment on a spacecraft. On Earth, such coatings could help reduce diseases transmitted from touching surfaces in aircraft cabins, health care facilities, public transportation, and other settings.

McArthur, wearing a red shirt, steadies herself with her left hand and aims a camera in her right hand at a rectangular panel on a wall of the space station labeled Boeing Antimicrobial Coating with 15 squares of different materials and colors.
NASA astronaut Megan McArthur documents touch panels installed for the ISS Boeing Antimicrobial Coating investigation.
NASA

Microbial Observatory-1 was one of the first investigations to monitor the types of microbes present on the space station. Researchers produced the genomes of multiple microorganisms, including some that may act as pathogens and cause disease. Published results include a comprehensive catalog of bacteria and fungi1 deposited into the NASA GeneLab system.

Kelly, wearing a red shirt and khaki pants, is in the center of a space station module reading from a sheaf of papers. In front of him is a blue square container, one of the microbial samplers. A large laptop screen is above Kelly and multiple cameras are attached to the wall to his left.
NASA astronaut Scott Kelly collects samples for Microbial Observatory-1.
NASA

The Microbial Tracking-2 investigation continued a series monitoring the types of microbes on the space station and attempted to catalog and characterize any with disease-causing potential. Researchers produced whole-genome sequences of 94 fungal strains2 and 96 bacterial strains of 14 species3. The data also revealed that Staphylococcus and Malassezia species were the most common bacteria and fungi, respectively, on the space station and that, overall, microorganisms associated with the human skin dominated the surface microbiome4.

iss052e006453~large.jpg?w=1920&h=1277&fi
A Microbial Tracking-2 device collects air samples.
NASA

BioRisk-MSV, a long-running Roscosmos investigation, examined physical and genetic changes in bacteria and fungi on interior and exterior surfaces of the space station. Researchers found that microorganisms not only survive in this extreme environment but retain their reproductive ability as well. Most microorganisms also exhibited increased biochemical activity and resistance to antibiotics5. These findings have implications for developing planetary quarantine methods and biomedical safety systems for future missions.

The TEST investigation from Roscomos examined samples from the exterior surface of the space station and in life support systems. This work demonstrated that it was possible to collect data on viable microorganisms from open space and identified specific non-spore-forming bacteria found there6. Researchers also found land and marine bacteria in cosmic dust samples collected during a spacewalk. These microbes may transfer from the upper atmosphere via the global electric circuit (a continuous movement of electric charge carriers such as ions) or they may have originated in space7.

NASA’s ISS External Microorganisms plans to continue this work, collecting samples near life support system vents outside the station to examine whether the spacecraft releases microorganisms and, if so, how many and how far they may travel.

Myco, an investigation from JAXA (Japan Aerospace Exploration Agency), evaluated whether fungi inhaled by crew members or that adhere to their skin can act as allergens. The data revealed an increased relative abundance of a common fungus associated with seborrheic dermatitis (an itchy skin rash), and the presence of several types of fungi not common on the skin8. Results also showed an abundance of a yeast that may have adhered to the skin of some crew members preflight, suggesting that a specific or uncommon microorganism can proliferate in a closed environment. This study was the first to reveal changes over time in the skin fungal microbiota of astronauts9.

Coleman is wearing a red shirt and blue pants, long brown hair floating around her head. She has thick white gloves on her hands and holds a silver rectangular container that she just took out of a refrigerator. Vapor rises from the container and refrigerator.
NASA astronaut Cady Coleman processes samples for the Myco Experiment.
NASA

JAXA also conducted a series of experiments, Microbe-I, Microbe-III, and Microbe-IV, monitoring the abundance and diversity of fungi and bacteria in Kibo, the station’s Japanese Experiment module. This work resulted in multiple publications reporting on the type and numbers of microorganisms detected10,11.

ISS Internal Environments provided a baseline of the contaminants on the space station. These data provide insight into the microbes present from the initial stages of construction through ongoing habitation of the orbiting lab.

This and other research on the microorganisms in and around the space station are helping to ensure that crew members remain in safe company on current and future missions.

John Love, ISS Research Planning Integration Scientist
Expedition 70

Citations:

1 Checinska Sielaff A, Urbaniak C, Mohan GB, Stepanov VG, Tran Q, Wood JM, Minich J, McDonald D, Mayer T, Knight R, Karouia F, Fox GE, Venkateswaran KJ. Characterization of the total and viable bacterial and fungal communities associated with the International Space Station surfaces. Microbiome. 2019 April 8; 7(1): 50. DOI: 10.1186/s40168-019-0666-x.

2 Simpson AC, Urbaniak C, Bateh JR, Singh NK, Wood JM, Debieu M, O’Hara NB, Houbraken J, Mason CE, Venkateswaran KJ. Draft genome sequences of fungi isolated from the International Space Station during the Microbial Tracking-2 experiment. Microbiology Resource Announcements. 2021 September 16; 10(37): e00751-21. DOI: 10.1128/MRA.00751-21.

3 Simpson AC, Urbaniak C, Singh NK, Wood JM, Debieu M, O’Hara NB, Mason CE, Venkateswaran KJ. Draft genome sequences of various bacterial phyla isolated from the International Space Station. Microbiology Resource Announcements. 2021 April 29; 10(17): e00214-21. DOI: 10.1128/MRA.00214-21.

4 Urbaniak C, Morrison MD, Thissen J, Karouia F, Smith DJ, Mehta SK, Jaing C, Venkateswaran KJ. Microbial Tracking-2, a metagenomics analysis of bacteria and fungi onboard the International Space Station. Microbiome. 2022 June 29; 10(1): 100. DOI: 10.1186/s40168-022-01293-0.

5 Sychev VN, Novikova ND, Poddubko SV, Deshevaya EA, Orlov OI. The biological threat: The threat of planetary quarantine failure as a result of outer space exploration by humans. Doklady Biological Sciences. 2020 January; 490(1): 28-30. DOI: 10.1134/S0012496620010093.PMID: 32342323. Russian Text © The Author(s), 2020, published in Doklady Rossiiskoi Akademii Nauk. Nauki o Zhizni, 2020, Vol. 490, pp. 105–108.

6 Deshevaya EA, Shubralova EV, Fialkina SV, Guridov AA, Novikova ND, Tsygankov OS, lianko PS, Orlov OI, Morzunov SP, Rizvanov AA, Nikolaeva IV. Microbiological investigation of the space dust collected from the external surfaces of the International Space Station. BioNanoScience. 2020 March 1; 10(1): 81-88. DOI: 10.1007/s12668-019-00712-1.

7 Grebennikova TV, Syroeshkin AV, Shubralova EV, Eliseeva OV, Kostina LV, Kulikova NY, Latyshev OE, Morozova MA, Yuzhakov AG, Zlatskiy IA, Chichaeva MA, Tsygankov OS. The DNA of bacteria of the world ocean and the Earth in cosmic dust at the International Space Station. The Scientific World Journal. 2018 20187360147. DOI: 10.1155/2018/7360147.

8 Sugita T, Yamazaki TQ, Cho O, Furukawa S, Mukai C. The skin mycobiome of an astronaut during a 1-year stay on the International Space Station. Medical Mycology. 2021 January; 59(1): 106-109. DOI: 10.1093/mmy/myaa067.PMID: 32838424.

9 Sugita T, Yamazaki TQ, Makimura K, Cho O, Yamada S, Ohshima H, Mukai C. Comprehensive analysis of the skin fungal microbiota of astronauts during a half-year stay at the International Space Station. Medical Mycology. 2016 March; 54(3): 232-239. DOI: 10.1093/mmy/myv121.

10 Yamaguchi N, Ichijo T, Nasu M. Bacterial monitoring in the International Space Station-“Kibo” based on rRNA gene sequence. Transactions of the Japan Society for Aeronautical and Space Sciences, Aerospace Technology Japan. 2016 14(ists30): Pp_1-Pp_4. DOI: 10.2322/tastj.14.Pp_1. 11 Satoh K, Alshahni MM, Umeda Y, Komori A, Tamura T, Nishiyama Y, Yamazaki TQ, Makimura K. Seven years of progress in determining fungal diversity and characterization of fungi isolated from the Japanese Experiment Module KIBO, International Space Station. Microbiology and Immunology. 2021 November; 65(11): 463-471. DOI: 10.1111/1348-0421.12931.

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      The SMAP project also produces L4 data that are the result of data assimilation. The L4 products take advantage of other environmental observations, such as precipitation, air temperature and humidity, radiative fluxes at the land surface, and ancillary land use and soil texture information, to produce estimates of surface [nominally 0–5 cm (0–2 in)] and subsurface (e.g., root-zone up to a meter) soil moisture. The data assimilation system is a merger of model and measurements and hence resolves the diurnal cycle of land surface conditions. The data assimilation system also provides estimates of surface fluxes of carbon, energy, and water, such as evaporation, runoff, gross primary productivity (GPP), and respiration. The difference between GPP and respiration is the net ecosystem exchange, which is the net source/sink of the carbon cycle over land.
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      The merged SMOS–SMAP passive L-band radiometry data allows the generation of global, near daily surface soil moisture estimates, which are required to resolve fast hydrologic processes, such as gravity drainage and recharge flux. These parameters are only partially resolved with the SMAP, with a two to three day data refresh rate. This product interpolates the multi-angular SMOS data to the SMAP 40º incident angle and uses all SMAP algorithms, including correction of waterbody impact on SMAP brightness temperature, and ancillary data for geophysical inversions to soil moisture and VOD, ensuring consistency. The combined SMAP–SMOS data product may not be available daily across locations, such as Japan, parts of China, and the Middle East, where RFI affects data collection.
      Table. Soil Moisture Active Passive suite of science products are available through the National Snow and Ice Data Center, one of NASA’s Distributed Active Archive Centers.
      Product Type Product description Resolution (Gridding) Granule Extent SPL1BTB Geolocated, calibrated brightness temperature in time order 36 km Half Orbit SPL1CTB_E Backus-Gilbert interpolated, calibrated brightness temperature in time order (9 km) Half Orbit SPL1CTB Geolocated, calibrated brightness temperature on Equal-Area Scalable Earth V2 (EASE2) grid 36 km Half Orbit SPL1CTB_E Backus-Gilbert interpolated, calibrated brightness temperature on EASE2 grid (9 km) Half Orbit SPL2SMP Radiometer soil moisture and vegetation optical depth 36 km Half Orbit SPL2SMP_E Radiometer soil moisture and vegetation optical depth based on SPL1CTB (9 km) Half Orbit SPL2SMAP_S SMAP radiometer/Copernicus Sentinel-1 soil moisture 3 km Sentinel-1 SPL3SMP Daily global composite radiometer soil moisture and vegetation optical depth based on SPL1CTB 36 km Daily–Global SPL3SMP_E Daily global composite radiometer soil moisture and vegetation optical depth based on SPL1CTB_E (9 km) Daily–Global SPL3FTP Daily composite freeze/thaw state based on SPL1CTB 36 km Daily–Global SPL3FTP_E Daily composite freeze/thaw state based on SPL1CTB_E (9 km) Daily–Global SPL4SMAU Surface and Root Zone soil moisture 9 km 3 hours – Global SPL4CMDL Carbon Net Ecosystem Exchange 9 km Daily–Global SPL1BTB_NRT Near Real Time Geolocated, calibrated brightness temperature in time order 36 km Half Orbit SPL2SMP_NRT Near Real Time Radiometer soil moisture 36 km Half Orbit L2/L3 SMOS SM SMOS soil moisture and VOD based on SMAP algorithms (9 km) Half Orbit/Daily Global Future Directions for the SMAP Active–Passive Algorithm
      Although the SMAP radar failed not long after launch, the data that were collected have been used to advance the development of the SMAP Active–Passive (AP) algorithm, which will be applied to the combined SMAP radiometer data and radar data from the NASA–Indian Space Research Organisation (ISRO) Synthetic Aperture Radar [NISAR] mission, a recently-launched L-Band Synthetic Aperture mission to produce global soil moisture at a spatial resolution of 1 km (0.62 mi) or better. The high resolution product can advance applications of SMAP data (e.g., agricultural productivity, wildfire, and landslide monitoring).
      Data Continuity Beyond SMAP
      A forthcoming mission meets some – but not all – of the SMAP measurement requirements and desired enhancements. The European Union’s Copernicus Program Copernicus Imaging Microwave Radiometer (CIMR) mission is a proposed multichannel microwave radiometry observatory that includes L-band and four other microwave channels sharing a large mesh reflector. The mesh reflector is similar to the one that is used on SMAP, but larger. The successful SMAP demonstration of rotating large deployable mesh antennas for Earth observations has been useful to the CIMR design.
      In terms of RFI detection capability, CIMR will also use an approach that is similar to SMAP. With regard to instrument thermal noise (NEDT) and data latency, CIMR meets or comes close to the next-mission desired characteristics and equals or exceeds SMAP in most of the attributes. The native L-band resolution of CIMR is ~60 km (37 mi); however, the measurements are coincident and higher-resolution measurements in this configuration allow reconstruction of L-band radiometry at higher resolution than CIMR’s L-band. It may be possible to combine the L- and C-bands and achieve a reconstructed ~15 km (9 mi) L-band product based on the coincident and overlapping measurements. A refresh rate of one day is possible with the wide-swath characteristic of CIMR.
      CIMR is currently in development; the first version, CIMR-1A, is expected to launch within this decade and the second version, CIMR-1B, in the mid 2030s. Since the Copernicus program supports operational activities (e.g., numerical weather prediction), the program includes plans for follow-on CIMR observatories so that the data record will be maintained without gaps in the future.
      Conclusions
      The SMAP mission was launched in 2015 and has produced over 10 years of science data. Because of its unique instrument and operating characteristics, the global low-frequency microwave radiometry with the SMAP observatory has resulted in surface soil moisture, vegetation optical depth, and freeze/thaw state estimates that outperform past and current products. The data have been widely used in the Earth system science community and also applied to natural hazards applications.
      The Earth system science and application communities are actively using the decade-long, high-quality global L-band radiometry. The intensity and range of SMAP science data usage is evident in the number of peer-reviewed journal publications that contain SMAP or Soil Moisture Active Passive in their title or abstract and use SMAP data in the study (i.e., search: www.webofscience.com data-base). The authors acknowledge that many publications escape this particular query approach. Currently the bibliography includes over 1700 entries and over 20,000 citations spanning several elements of Earth system science, including hydrologic science and regional and global water cycle, oceanic and atmospheric sciences, cryosphere science, global ecology as well as microwave remote sensing technologies.
      To Learn More About SMAP
      A more comprehensive bibliography of studies published based on SMAP data products, a set of one-page SMAP science and applications highlights in standardized format, and SMAP project documents including assessment reports are all available online via the links provided.
      Acknowledgements
      The authors wish to acknowledge the contributions of the SMAP Science Team, the SMAP Algorithm Development Team, and the SMAP Project Office engineers and staff. All of these teams contribute to the ongoing SMAP science product generation and uses reported in this article.
      Dara Entekhabi
      Massachusetts Institute of Technology
      darae@mit.edu
      Simon Yueh
      Jet Propulsion Laboratory/California Institute of Technology
      simon.h.yueh@jpl.nasa.gov
      Rajat Bindlish
      NASA Goddard Space Flight Center
      rajat.bindlish@nasa.gov
      Mark Garcia
      Jet Propulsion Laboratory/California Institute of Technology
      mark.d.garcia@jpl.nasa.gov
      Jared Entin
      NASA Headquarters
      jared.k.entin@nasa.gov
      Craig Ferguson
      NASA Headquarters
      craig.r.ferguson@nasa.gov
      Share








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      Last Updated Aug 18, 2025 Related Terms
      Earth Science View the full article
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      The next sea-level monitoring satellite, Copernicus Sentinel-6B, has begun its journey from Europe to the Vandenberg Space Force Base in California, where it is scheduled to launch in November. Carefully packed into a climate-controlled container, the satellite is currently crossing the Atlantic Ocean aboard the cargo ship Industrial Dolphin.
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      Since launching in 2023, NASA’s Tropospheric Emissions: Monitoring of Pollution mission, or TEMPO, has been measuring the quality of the air we breathe from 22,000 miles above the ground. June 19 marked the successful completion of TEMPO’s 20-month-long initial prime mission, and based on the quality of measurements to date, the mission has been extended through at least September 2026. The TEMPO mission is NASA’s first to use a spectrometer to gather hourly air quality data continuously over North America during daytime hours. It can see details down to just a few square miles, a significant advancement over previous satellites.
      “NASA satellites have a long history of missions lasting well beyond the primary mission timeline. While TEMPO has completed its primary mission, the life for TEMPO is far from over,” said Laura Judd, research physical scientist and TEMPO science team member at NASA’s Langley Research Center in Hampton, Virginia. “It is a big jump going from once-daily images prior to this mission to hourly data. We are continually learning how to use this data to interpret how emissions change over time and how to track anomalous events, such as smoggy days in cities or the transport of wildfire smoke.” 
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      By measuring nitrogen dioxide (NO2) and formaldehyde (HCHO), TEMPO can derive the presence of near-surface ozone. On Aug. 2, 2024 over Houston, TEMPO observed exceptionally high ozone levels in the area. On the left, NO2 builds up in the atmosphere over the city and over the Houston Ship Channel. On the right, formaldehyde levels are seen reaching a peak in the early afternoon. Formaldehyde is largely formed through the oxidation of hydrocarbons, an ingredient of ozone production, such as those that can be emitted by petrochemical facilities found in the Houston Ship Channel. Trent Schindler/NASA's Scientific Visualization Studio When air quality is altered by smog, wildfire smoke, dust, or emissions from vehicle traffic and power plants, TEMPO detects the trace gases that come with those effects. These include nitrogen dioxide, ozone, and formaldehyde in the troposphere, the lowest layer of Earth’s atmosphere.
      “A major breakthrough during the primary mission has been the successful test of data delivery in under three hours with the help of NASA’s Satellite Needs Working Group. This information empowers decision-makers and first responders to issue timely air quality warnings and help the public reduce outdoor exposure during times of higher pollution,” said Hazem Mahmoud, lead data scientist at NASA’s Atmospheric Science Data Center located at Langley Research Center.
      …the substantial demand for TEMPO's data underscores its critical role…
      hazem mahmoud
      NASA Data Scientist
      TEMPO data is archived and distributed freely through the Atmospheric Science Data Center. “The TEMPO mission has set a groundbreaking record as the first mission to surpass two petabytes, or 2 million gigabytes, of data downloads within a single year,” said Mahmoud. “With over 800 unique users, the substantial demand for TEMPO’s data underscores its critical role and the immense value it provides to the scientific community and beyond.” Air quality forecasters, atmospheric scientists, and health researchers make up the bulk of the data users so far.
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      On April 14, strong winds triggered the formation of a huge dust storm in the U.S. central plains and fueled the ignition of grassland fires in Oklahoma. On the left, the NO2 plumes originating from the grassland fires are tracked hour-by-hour by TEMPO. Smoke can be discerned from dust as a source since dust is not a source of NO2. The animation on the right shows the ultraviolet (UV) aerosol index, which indicates particulates in the atmosphere that absorb UV light, such as dust and smoke. Trent Schindler/NASA's Scientific Visualization Studio The TEMPO mission is a collaboration between NASA and the Smithsonian Astrophysical Observatory, whose Center for Astrophysics Harvard & Smithsonian oversees daily operations of the TEMPO instrument and produces data products through its Instrument Operations Center.
      Datasets from TEMPO will be expanded through collaborations with partner agencies like the National Oceanic and Atmospheric Administration (NOAA), which is deriving aerosol products that can distinguish between smoke and dust particles and offer insights into their altitude and concentration.
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      On May 5, TEMPO measured NO2 emissions over the Twin Cities in the center of Minnesota during morning rush hour. The NO2 increases seen mid-day through the early evening hours are illustrated by the red and black shaded areas at the Red River Valley along the North Dakota state line. These levels are driven by emissions from the soils in agriculturally rich areas. Agricultural soil emissions are influenced by environmental factors like temperature and moisture as well as fertilizer application. Small fires and enhancements from mining activities can also be seen popping up across the region through the afternoon.Trent Schindler/NASA's Scientific Visualization Studio “These datasets are being used to inform the public of rush-hour pollution, air quality alerts, and the movement of smoke from forest fires,” said Xiong Liu, TEMPO’s principal investigator at the Center for Astrophysics Harvard & Smithsonian. “The library will soon grow with the important addition of aerosol products. Users will be able to use these expanded TEMPO products for air quality monitoring, improving forecast models, deriving pollutant amounts in emissions and many other science applications.”
      The TEMPO mission detects and highlights movement of smoke originating from fires burning in Manitoba on June 2. Seen in purple hues are observations made by TEMPO in the ultraviolet spectrum compared to Advanced Baseline Imagers (ABIs) on NOAA’s GOES-R series of weather satellites that do not have the needed spectral coverage. The NOAA GOES-R data paired with NASA’s TEMPO data enhance state and local agencies’ ability to provide near-real-time smoke and dust impacts in local air quality forecasts.NOAA/NESDIS/Center for Satellite Applications and Research “The TEMPO data validation has truly been a community effort with over 20 agencies at the federal and international level, as well as a community of over 200 scientists at research and academic institutions,” Judd added. “I look forward to seeing how TEMPO data will help close knowledge gaps about the timing, sources, and evolution of air pollution from this unprecedented space-based view.”
      An agency review will take place in the fall to assess TEMPO’s achievements and extended mission goals and identify lessons learned that can be applied to future missions.
      The TEMPO mission is part of NASA’s Earth Venture Instrument program, which includes small, targeted science investigations designed to complement NASA’s larger research missions. The instrument also forms part of a virtual constellation of air quality monitors for the Northern Hemisphere which includes South Korea’s Geostationary Environment Monitoring Spectrometer and ESA’s (European Space Agency) Sentinel-4 satellite. TEMPO was built by BAE Systems Inc., Space & Mission Systems (formerly Ball Aerospace). It flies onboard the Intelsat 40e satellite built by Maxar Technologies. The TEMPO Instrument Operations Center and the Science Data Processing Center are operated by the Smithsonian Astrophysical Observatory, part of the Center for Astrophysics | Harvard & Smithsonian in Cambridge.


      For more information about the TEMPO instrument and mission, visit:
      https://science.nasa.gov/mission/tempo/

      About the Author
      Charles G. Hatfield
      Science Public Affairs Officer, NASA Langley Research Center
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      The Cardi/o vital sign monitor uses NASA-developed technology to continually monitor vital signs. The data collected can be sent directly to medical care providers, cutting down on the number of home healthcare visits. Credit: Advanced TeleSensors Inc. The Cardi/o Monitor is 3 inches square and mounts to a ceiling or wall. It can detect vital signs from up to 10 feet. Multiple devices can be scattered throughout a house, with a smartphone app controlling settings and displaying all data on a single dashboard. The algorithms NASA developed detect heartbeat and respiration, and the company added heart rate variability detection that indicates stress and sleep apnea.  

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      Through the commercialization of this life-preserving technology, NASA is at the heart of advancing health solutions.  
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