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Add-on to Large-Scale Water Mining Operations on Mars to Screen for Introduced and Alien Life


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Preparations for Next Moonwalk Simulations Underway (and Underwater)

Artist rendition of labled Water mining ISRU missins on a lunar surface.
Graphic depiction of Add-on to large-scale water mining operations on Mars to screen for introduced and alien life
Steven Benner

Steven Benner
Foundation For Applied Molecular Evolution

As noted at NASA’s 2019 Carlsbad Conference we have good reason to think that:

  • Life started on Mars using the same geo-organic chemistry that started life on Earth.
  • Martian life persists today on Mars, in near-surface ice, low elevations, and caves, all with transient liquid brines, environments that today on Earth host microbial life.
  • Martian life must use informational polymers (like DNA); Darwinian evolution requires these, and Darwinian evolution is the only way matter can organize to give life.
  • While Martian “DNA” may differ (possibly radically) in its chemistry from Terran DNA, the “Polyelectrolyte Theory of the Gene” limits the universe of possible alien DNA structures.
  • Those structures ensure that Martian DNA can be concentrated from Martian water, even if very highly diluted, and even if Martian “DNA” differs from Earth DNA.
  • On Mars as it exists today, information polymers cannot be generated without life (unlike other less reliable biosignatures such as methane), ensuring that life will not be “detected” if it is not present (the “false positive problem”). Nevertheless, as noted by Rummel and Conley, “the Mars community is not convinced that a mission to attempt detection of extant Martian life has a high priority.” Thus, NASA’s current flagship mission to Mars, derived from its 2012 Decadal Survey, involves pedestrian collection of old dry rocks to be cached, eventually to be returned to Earth to study for evidence of past life.

The purpose of this NIAC project is to change this view, and to do so before human arrival planned by NASA, the Chinese National Space Agency, and SpaceX, “by 2040”, “in 2033”, and “before 2030”, according to their respective statements. Human arrival will undoubtedly complicate the search for indigenous Martian life. Thus, from an astrobiological perspective, these planned crewed missions to Mars put a very strict deadline on the search for life on a pristine Mars. However, crewed missions also offer an opportunity that we will exploit. Crewed missions to Mars will use materials found on Mars itself, “in situ”, in particular, near surface water ice. Propellant (methane and oxygen) will be generated from that water and

atmospheric carbon dioxide for the return trip back to Earth. That water ice will be mined on the scale of tens to hundred tons. Further, to maximize the likelihood of safe return of the crew to Earth, robotic operations that mine tons of near surface water-ice will be in place before the first human astronauts arrive. Thus, water mined in preparation for human arrival is correctly seen as an extremely large-scale astrobiological sample, far larger than dry cached rocks. As the mined water-ice is delivered with dust that, through dust storms, survey the entire accessible surface, this humongous sample will effectively enable a highly sensitive survey of the entire accessible Mars surface for life. This NIAC project will provide an “agnostic life finding” (ALF) system capable of extracting genetic polymers (DNA or alien) from

these large ISRU water samples. ALF is agnostic because it exploits what synthetic biology taught us about the limited kinds of Darwinian genetic molecules. ALF also offers tools to partly analyze the polyelectrolytes in situ.

As an add-on system, ALF creates a negligible additional burden (regarding mass and energy consumption) compared to the investment in the water mining operation at this scale. Although small and low cost, this instrument will allow science to place a severe lower limit on the amount of biosphere on the accessible Martian surface. And it will do so before Homo sapiens becomes a multiplanetary species. And “multiplanetary” is the correct term. This add-on ALF system can be used on all celestial bodies where water will be mined to search for and analyze life, indigenous or introduced, Earth-like or alien. This includes Europa, Enceladus, the Moon, and exotic locales on Earth.

2024 Phase I Selection

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      The frigid surfaces of these nearly airless moons are likely uninhabitable due to radiation from both high-speed particles trapped in their host planet’s magnetic fields and powerful events in deep space, such as exploding stars. However, both have oceans under their icy surfaces that are heated by tides from the gravitational pull of the host planet and neighboring moons. These subsurface oceans could harbor life if they have other necessities, such as an energy supply as well as elements and compounds used in biological molecules.
      Dramatic plumes, both large and small, spray water ice and vapor from many locations along the famed “tiger stripes” near the south pole of Saturn’s moon Enceladus. NASA/JPL/Space Science Institute The research team used amino acids in radiolysis experiments as possible representatives of biomolecules on icy moons. Amino acids can be created by life or by non-biological chemistry. However, finding certain kinds of amino acids on Europa or Enceladus would be a potential sign of life because they are used by terrestrial life as a component to build proteins. Proteins are essential to life as they are used to make enzymes which speed up or regulate chemical reactions and to make structures. Amino acids and other compounds from subsurface oceans could be brought to the surface by geyser activity or the slow churning motion of the ice crust.
      This view of Jupiter’s icy moon Europa was captured by JunoCam, the public engagement camera aboard NASA’s Juno spacecraft, during the mission’s close flyby on Sept. 29, 2022. The picture is a composite of JunoCam’s second, third, and fourth images taken during the flyby, as seen from the perspective of the fourth image. North is to the left. The images have a resolution of just over 0.5 to 2.5 miles per pixel (1 to 4 kilometers per pixel).
      As with our Moon and Earth, one side of Europa always faces Jupiter, and that is the side of Europa visible here. Europa’s surface is crisscrossed by fractures, ridges, and bands, which have erased terrain older than about 90 million years.
      Citizen scientist Kevin M. Gill processed the images to enhance the color and contrast.
      NASA/JPL-Caltech/SwRI/MSSS Image processing: Kevin M. Gill CC BY 3.0 To evaluate the survival of amino acids on these worlds, the team mixed samples of amino acids with ice chilled to about minus 321 Fahrenheit (-196 Celsius) in sealed, airless vials and bombarded them with gamma-rays, a type of high-energy light, at various doses. Since the oceans might host microscopic life, they also tested the survival of amino acids in dead bacteria in ice. Finally, they tested samples of amino acids in ice mixed with silicate dust to consider the potential mixing of material from meteorites or the interior with surface ice.
      This image shows experiment samples loaded in the specially designed dewar which will be filled with liquid nitrogen shortly after and placed under gamma radiation. Notice that the flame-sealed test tubes are wrapped in cotton fabric to keep them together because test tubes become buoyant in liquid nitrogen and start floating around in the dewar, interfering with the proper radiation exposure. Candace Davison The experiments provided pivotal data to determine the rates at which amino acids break down, called radiolysis constants. With these, the team used the age of the ice surface and the radiation environment at Europa and Enceladus to calculate the drilling depth and locations where 10 percent of the amino acids would survive radiolytic destruction.
      Although experiments to test the survival of amino acids in ice have been done before, this is the first to use lower radiation doses that don’t completely break apart the amino acids, since just altering or degrading them is enough to make it impossible to determine if they are potential signs of life. This is also the first experiment using Europa/Enceladus conditions to evaluate the survival of these compounds in microorganisms and the first to test the survival of amino acids mixed with dust.
      The team found that amino acids degraded faster when mixed with dust but slower when coming from microorganisms.
      “Slow rates of amino acid destruction in biological samples under Europa and Enceladus-like surface conditions bolster the case for future life-detection measurements by Europa and Enceladus lander missions,” said Pavlov. “Our results indicate that the rates of potential organic biomolecules’ degradation in silica-rich regions on both Europa and Enceladus are higher than in pure ice and, thus, possible future missions to Europa and Enceladus should be cautious in sampling silica-rich locations on both icy moons.”
      A potential explanation for why amino acids survived longer in bacteria involves the ways ionizing radiation changes molecules — directly by breaking their chemical bonds or indirectly by creating reactive compounds nearby which then alter or break down the molecule of interest. It’s possible that bacterial cellular material protected amino acids from the reactive compounds produced by the radiation.
      The research was supported by NASA under award number 80GSFC21M0002, NASA’s Planetary Science Division Internal Scientist Funding Program through the Fundamental Laboratory Research work package at Goddard, and NASA Astrobiology NfoLD award 80NSSC18K1140.
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      Last Updated Jul 18, 2024 Editor wasteigerwald Contact wasteigerwald william.a.steigerwald@nasa.gov Location NASA Goddard Space Flight Center Related Terms
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